Please thoroughly review core policies prior to submitting samples or initiating a project. If you have any questions regarding these policies or questions related to services, please contact core staff at (mugenomicscore@missouri.edu).
Sample Submission - The general guidelines for submitting samples are provided on our website: Sequencing Submission Guidelines. Researchers are responsible to ensure that samples have been prepared to the Genomics Core specifications.
Turnaround Time - All samples are handled on a first come first serve basis. The Genomics Core provides sequencing data in a turn around time of 24 hours. Turnaround time is defined as the period between the physical submission of a sample at the facility and the upload of results to the dnaLIMS server. Only business days (Monday through Friday) are used in determining turnaround time; university holidays excluded.
Deliverables - Data is returned as a chromatogram (.ab1) and text (.seq) file. Good quality template will provide ~1,100 bases of quality sequence data. While the Genomics Core strives to provide quality sequence for every sample submitted, we cannot be responsible for poor results due to factors outside of our control (i.e., secondary structure, poorly annealing primers, improperly submitted samples, etc.). If it is determined that a failed reaction was caused by instrument malfunction or a chemistry issue, the reaction will be repeated at no charge. Otherwise, all other reactions, including failed reactions, are billed at full cost.
Data Retention - Data is made available via the web through a secure login on the Genomics Core's dnaLIMS server. Sequence data is maintained for 6 months by the Genomics Core after which data will be deleted and no longer available. Researchers are strongly encouraged to download and review data as soon as possible once the run is completed.
Billing
Sample Submission - The general guidelines for submitting samples are provided on our website: Fragment Analysis Submission Guidelines. Researchers are responsible to ensure that samples have been prepared to Genomics Core specifications.
Turnaround Time - All samples are handled on a first come first serve basis. The Genomics Core provides fragment analysis data in an average turnaround time of 48-36 hours. Whole plate submissions can be processed in 24 hours. Turnaround time is defined as the period between the physical submission of a sample at the facility and the upload of results to the dnaLIMS server. Only business days (Monday through Friday) are used in determining turnaround time; university holidays excluded.
Deliverables - Data is returned as an Applied Biosystems genetic analysis data format file(.fsa). An internal lane standard is included in each lane providing a quality control for electrophoresis. While the Genomics Core strives to provide quality fragment sizing for every sample submitted, we cannot be responsible for poor results due to factors outside of our control (i.e., incorrect dye usage, improper post-PCR cleanup, etc.). If it is determined that a failed reaction was caused by instrument malfunction or a chemistry issue, the reaction will be repeated at no charge. Otherwise, all other reactions, including failed reactions, are billed at full cost.
Data Retention - Data is made available via the web through a secure login on the Genomics Core's dnaLIMS server. Fragment analysis data is maintained for 6 months by the Genomics Core after which data will be deleted and no longer available. Researchers are strongly encouraged to download and review data as soon as possible once the run is completed.
Billing - Billing policy is the same as outlined under Sanger sequencing services.
Note: All 96-well plates are billed as an entire plate regardless if the plate is partially filled.
Sample Submission - The general guidelines for submitting samples are provided on our website: Guidelines for Illumina Project Initiation. These are general recommendations but input amounts can vary. Discuss input requirements with core staff. Researchers are responsible to ensure that samples have been prepared to Genomics Core final specifications.
Run Schedule - Libraries are not scheduled for sequencing on Illumina instruments until submitted to the Genomics Core. Reservation of instrument time is not accepted.
Deliverables
Exceptions: The Genomics Core strives to successfully sequence all samples generating high quality data. However, the core cannot be responsible for improperly prepared samples. Therefore, we make no guarantees on data quality or data output, if:
Projects are billed in these situations, even with poor yields or failed results. If it is determined that a failed run was caused by instrument malfunction or a chemistry issue, the sequencing will be repeated at no charge.
Data Retention - Disk storage used by the Genomics Core is provided by Research Support Computing within the Division of IT. Although it is impractical to store all data indefinitely, the intention of the Genomics Technology Core is to maintain relevant data for a necessary period of time to enable its review and analysis. The Genomics Technology Core will retain data generated from Illumina instruments according to the following schedule.
Researchers are strongly encouraged to download and review data as soon as they receive their data link.
Billing