10x Genomics Visum Spatial Transcriptome Calculator
Calculator will help determine the correct flow cell selection to achieve necessary read depth on a Visium Spatial project.
The sequencing parameters and number of cycles required for the different single cell applications can be found on the core's 10x Genomic page.
*A ratio of 1.0 indicates a 100% usage of the flow cell. This would be the best match of flow cell to output need for the sequencing.
Assumptions used in calculations:
- NovaSeq S1 flow cell = 800 million paired reads
- NovaSeq S1 flow cell = 1.3 billion paired reads
- NovaSeq S2 flow cell = 3.3 billion paired reads
- NovaSeq S4 flow cell (lane) = 2.5 billion paired reads
- NovaSeq S4 flow cell = 10 billion paired reads