10x Genomics Single Cell Flow Cell Calculator

10x Genomics Visum Spatial Transcriptome Calculator



Calculator will help determine the correct flow cell selection to achieve necessary read depth on a Visium Spatial project.

The sequencing parameters and number of cycles required for the different single cell applications can be found on the core's 10x Genomic page.



Number of Array Tiles (4 tiles per slide)        

Percentage of Area Covered                             (enter as value between 0 and 1; ex. 60% = 0.6)

Targeted Reads Per Cell   =   50,000 read pairs per spot


                    Flow Cell Use Ratio*
NovaSeq SP Flow Cell              
NovaSeq S1 Flow Cell              
NovaSeq S2 Flow Cell              
NovaSeq S4 Lane     
NovaSeq S4 Flow Cell      

*A ratio of 1.0 indicates a 100% usage of the flow cell. This would be the best match of flow cell to output need for the sequencing.

Assumptions used in calculations: