MiSeq

The Illumina MiSeq is a DNA sequencing platform capable of generating paired-end, 250 base read lengths. The single lane flow cell design, on-board clustering and five minute cycle times allow for rapid sequencing times(~48 hours for a 2x250 run).


Instrument Specifications
  • Standard sequence read lengths of 75, 150, 250 and 300 bases
  • Single or paired-end reads
  • 25 million quality reads per lane
  • Maximum data yield of ~15 Gb
  • Greater than 70% of bases >Q30 on a 2x300 run
Applications
  • Amplicon sequencing - The longer read lengths allow for paired-end sequencing which can be used to build contigs of PCR amplicons of up to 475 bases in length.
  • Metagenomics - The longer read lengths aid in sequencing of the hypervariable regions of the 16S RNA gene to characterize microbial communities.
  • Bacterial genome resequencing - The 8Gb yield of a 2x250 run is sufficient data yield to sequence bacterial genomes.

Sequencing Fees

.....Analysis Description.... USD Data Output (Gb)
Paired-End Read (2x75 bases) $1,275 / flow cell ~3.8Gb / flow cell
Paired-End Read (2x150 bases) $1,417 / flow cell ~4.5Gb / flow cell
Paired-End Read (2x250 bases) $1,576 / flow cell ~8Gb / flow cell
Paired-End Read (2x300 bases) $2,038 / flow cell ~15Gb / flow cell
Paired-End Read (2x150 bases), Nano** $700 / flow cell ~300Mb / flow cell
Paired-End Read (2x250 bases), Nano** $755 / flow cell ~500Mb / flow cell


*Contact the DNA Core for external rates

**Low yield kits primarily used for library QC runs