Library preparation services are offered by the DNA Core with more than 1000 libraries constructed in-house annually . Staff are experienced in constructing libraries from a varitey of sample inputs:
- total RNA
- enriched small RNA
- enriched mRNA
- immuno-precipitated DNA(ChIP-Seq)
- genomic DNA
Non-standard library construction requests will be considered and accepted on an individual basis.
Researchers may submit libraries prepared in their own labs. The DNA Core recommends researchers discuss with core staff the preparation of libraries to ensure successful sequencing. Researcher prepared libraries have a typical sequencing turnaround time of 1-2 weeks.
The Illumina sequencing platform has the capability to pool samples in a single lane to reduce the cost of sequencing. Sample pooling, or multiplexing, is accomplished with a barcode sequence incorporated in to each fragment during library construction that is used to sort reads post-run. The DNA Core currently supports the Illumina indexing system for core prepared libraries. The Illumina indexing system allows the pooling of up to 96 samples per lane for RNA-Seq and genomic libraries, and 48 samples for small RNA libraries. Alternate indexing approaches are available to researchers requiring higher multiplexing capabilities. Consult with the DNA Core if you are considering a multiplexing approach which does not use Illumina indexing.
Contact the DNA Core for additional information.