Library preparation services are offered by the DNA Core. Staff are experienced in constructing libraries from total RNA, enriched small RNA, enriched mRNA, immuno-precipitated DNA(ChIP-Seq), amplicons, and genomic DNA with more than 1,000 libraries prepared in-house annually. Non-standard library construction requests will be considered and accepted on an individual basis.
Researchers may submit libraries prepared in their own labs. The DNA Core recommends researchers discuss with core staff the preparation of libraries to ensure successful sequencing. Researcher prepared libraries have a typical turn-around time of 2-4 weeks.
Library Construction Fees
|Total RNA Analysis Fee||$7||Sample|
|NGS Fragment Analysis Fee (HS)||$7||Sample|
|Qubit Quantitation Fee||$3.50||Sample|
|Genomic Library Preparation (TruSeq PCR-free)||$200||Sample|
|ChIP-Seq Library Preparation (NEB Ultra DNA)||$180||Sample|
|RNA-Seq Library Preparation (TruSeq mRNA Stranded)||$200||Sample|
|Small RNA Library Preparation||$240||Sample|
|RNA-Seq Library Preparation(rRNA depletion) *bacterial||$275||Sample|
|Ultra Low RNA Library Preparation (Clonetech)||$375||Sample|
|WGBS Library Preparation (NEB)||$220||Sample|
|Methylated DNA Enrichment Library Preparation (NEB)||$220||Sample|
|16S Sequencing (V4 amplicon)||Contact DNA Core|
The Illumina sequencing platform has the capability to pool samples in a single lane to reduce the cost of sequencing. Sample pooling, or multiplexing, is accomplished with a barcode sequence incorporated in to each fragment during library construction that is used to sort reads post-run (see table below). The DNA Core currently supports the Illumina indexing system for core prepared libraries. The Illumina indexing system allows the pooling of up to 24 samples per lane for RNA-Seq and genomic libraries, and 48 samples for small RNA libraries. Alternate indexing approaches are available to researchers requiring higher multiplexing capabilities. Consult with the DNA Core if you are considering a multiplexing approach which does not use Illumina indexing.