NextSeq 500

The Illumina NextSeq 500 provides high-throughtput sequencing at increased speeds and lower costs. The platform is capable of generating 120Gb of data (~800 million paired-end reads) in a single run. The system is compatible with all Illumina libraries. The NextSeq 500 has the ability to perform whole genome sequencing but is also well suited for transciptome sequencing, exome sequencing and targeted resequencing. The faster run times are achieved with use of Illumina's new two-channel SBS technology which requires only two images to capture all four base calls.

Review the following Illumina technology notes to understand the difference between two-channel and four-channel SBS sequencing.     Learn More »

Contact the DNA Core to discuss with core staff how the NextSeq 500 can be used to generate sequence data for your project.


Instrument Specifications
  • Standard sequence read lengths of 75 and 150 bases.
  • Single or paired-end reads
  • ~800 million quality paired-end reads per lane
  • Maximum data yield of 120Gb per flow cell
  • Greater than 75% of bases >Q30 on a 2x150 run





Sequencing Fees

.....Analysis Description.... USD Data Output (Gb)
Single Read (75 bases) - HighOutput $1,835 / lane ~30Gb / lane
Single Read (150 bases) - HighOutput $3,465 / lane ~60Gb / lane
Paired-End Read (2x75 bases) - HighOutput $3,465 / lane ~60Gb / lane
Paired-End Read (2x150 bases) - HighOutput $5,165 / lane ~120Gb / lane
Paired-End Read (2x75 bases) - MidOutput $1,590 / lane ~18Gb / lane
Paired-End Read (2x150 bases) - MidOutput $2,240 / lane ~35Gb / lane

*Contact the DNA Core for external rates