RNA-Seq Project Calculator
Calculator will determine the number of total reads and the correct flow cell selection to achieve necessary read depth on a RNA-Seq project. Inquire about our NovaSeq Shared Services to take advantage of the NovaSeq throughput.
Typical RNA-Seq parameters.
- 50 million paired reads per sample for a mammalian size transcriptome
- 20 million paired reads per sample for bacterial transcriptome
Note: The flow cell closest to a whole number (i.e., 1, 2, 3,...) is the best match for the sequencing project.
Assumptions used in calculations:
- NovaSeq S1 Flow Cell = 800 million paired reads
- NovaSeq S1 Flow Cell = 1.3 billion paired reads
- NovaSeq S2 Flow Cell = 3.3 billion paired reads
- NovaSeq S4 Lane = 2.5 billion paired reads
- NovaSeq S4 Flow Cell = 10 billion paired reads