In 2019, Dr. Wes Warren received an award through the UM System Research and Creative Works Strategic Investment Program , a component of the University of Missouri System Strategic Plan approved by the Board of Curators to invest toward the five Missouri Compacts for Excellence. The Tier 1 award funded the purchase of an Illumina NovaSeq system currently housed in the DNA Core Facility.
As a central campus/system resource, the NovaSeq is having a broad impact on the development of cross-campus and system research networks and will continue to provide a sustainable resource central to new Program, Centers of Excellence and other large multi-PI externally funded programs and thus will impact all levels of multi-disciplinary basic, biomedical and clinical research envisioned by University leaders. Matching funds for the purchase of the NovaSeq were secured from the Office of Research, School of Medicine, College of Arts and Science, College of Agriculture, Food and Natural Resources, College of Veterinary Medicine and the Bond Life Sciences Center.
In addition to the matching funds, MU schools have allocated at varying levels funds to be used toward block grants to be awarded for NovaSeq sequencing. MU schools having allocated a higher matching amount will have a larger portion of sequencing capacity at the discounted rates. These block grants are expected to energize MU research funding. A review committee with proportional school representation will oversee the distribution of funds.
Block Grants Available in Year 1
School/College Source Number Amount College of Agriculture, Food and Natural Resources 5 up to $8,000       College of Arts and Science 4 up to $8,000       College of Veterinary Medicine 5-10 up to $10,000       School of Medicine 10-20 up to $20,000
The following are examples of typical sequencing costs for methods/applications at varying project scales. Costs are only examples for researchers to consider in planning a grant proposal. A final budget should involve contacting Nathan Bivens (BivensN@missouri.edu), Director - DNA Core Facility, to confirm final library preparation and NovaSeq costs.
Bulk RNA-Seq * assume 50 million paired reads per sample
Run Type Run Cost Sample Number Cost per Sample NovaSeq SP PE 50 $3,340 16 $208.75 NovaSeq SP PE 100 $4,003 16 $250.18 NovaSeq S1 PE 100 $6,145 27 $227.59 NovaSeq S2 PE 100 $10,368 68 $152.47 NovaSeq S4 PE 100 $14,382 192 $74.90
Whole genome sequencing * assumes 30x coverage per human genome (2.9 Gb)
Run Type Run Cost Sample Number Cost per Sample NovaSeq SP PE 150 $4,258 3 $1,419.33 NovaSeq S1 PE 150 $6,553 6 $1,092.16 NovaSeq S2 PE 150 $10,990 15 $732.66 NovaSeq S4 PE 150 $15,886 32 $496.43
single cell RNA-seq * assumes 50,000 reads per cell across 5,000 cells per sample
Run Type Run Cost Sample Number Cost per Sample NovaSeq SP PE 50 $3,340 3 $1,113.33 NovaSeq S1 PE 50 $5,125 5 $1025.00 NovaSeq S2 PE 50 $8,593 14 $613.78 NovaSeq S4 PE 50 $14,382 40 $359.55
Exome sequencing * assumes 100x coverage
Run Type Run Cost Sample Number Cost per Sample NovaSeq SP PE 100 $4,003 20 $200.15 NovaSeq S1 PE 100 $6,145 50 $122.90 NovaSeq S2 PE 100 $10,368 150 $69.12
- Grant block funding sizes and numbers specific to each contributing school/college will be announced via the DNA core web site. The investigator's primary appointment will determine which school/college to search for specific funding information. A start date of when sequencing applications are accepted will be posted to start the submission process.
- Applications are downloaded by investigator, filled out with the appropriate information as described in the example grant for each required category. Point totals for each category will be defined in the application document.
- At the end of 1 month, starting when the grant application date opens, the committee will review the total of proposals received. A score will be assigned with a maximum of 100 points awarded. Since each school/college has a set amount of grants to be established by the committee it is expected the block funds available will vary across schools/colleges. We have provided funding flexibility within the committee to decide on a final number of grants that is most appropriate for the investigators.
- The funding will be dispersed in grant blocks until funds are depleted by school/college. Therefore, we have a first come basis for funding throughout the year. If after the initial review of grants funds still remain, we will announce this fact on the DNA Core web site.
- If funds are not awarded in a first application investigators can amend their proposal and resubmit for consideration in the next review cycle. This assumes Year 1 funds remain after the initial review. It is encouraged that each investigator in this situation seek out feedback from their committee representative prior to resubmission.
Merit review criteria will be similar to those used by Federal granting agencies such as NIH and NSF. These include scientific and technical significance, innovation, approach, and likelihood of success of achieving external funding. Proposals will only be considered to cover the costs of sequencing on the NovaSeq 6000 platform, and not related expenses such as library preparation, tissue collection, or salary support. The following criteria will be considered by the grant review committee:
- Study introduction and rationale: Proposals must document specific evidence of informatics and data analysis capabilities – either through in-house expertise or collaboration – to obtain meaningful insights from the data generated (20 pts).
- Experimental design or methods: Proposals should describe the number of samples to be sequenced, at what depth, and what technology to be used, e.g. WGS, WES, RNAseq, etc. A summary of the experimental design ought to be enough to convince reviewers the study design will produce the predicted outcome (50 pts).
- Budget: Proposals must present a realistic and accurate assessment of the appropriate scope of work and budget necessary to generate data sufficient to achieve the proposed goals of the project in an efficient and cost-effective manner. Special consideration will be given to those proposals that make use of matching funds or other resources to increase the impact of the sequencing data (10 pts).
- Goals and Data Use: Proposals need to describe how the sequencing data will specifically facilitate the development and submission of a proposal(s) seeking external funding, as well as the expectations regarding the impact on the research program/question of the PI. Any intended use of the data that will result in possible publications should also be described as appropriate and will be considered a strength (20 pts).
|         ||Wes Warren||         ||Professor Genomics||         ||CAFNR|| |  firstname.lastname@example.org|
|         ||   ||         ||Division of Animal Sciences||         ||         |
|         ||Robert Schnabel||         ||Associate Professor||         ||CAFNR|| |  email@example.com|
|         ||   ||         ||Division of Animal Sciences||         ||         |
|         ||David Schulz||         ||Director of Biological Sciences||         ||College of Arts and Science|| |  firstname.lastname@example.org|
|         ||   ||         ||Division of Biological Sciences||         ||      ||         |
|         ||Leslie Lyons||         ||Endowed Professor of Comparative Medicine||         ||College of Veterinary Medicine|| |  lyonsla@Missouri.edu|
|         ||   ||         ||Veterinary Medicine & Surgery||      ||         |
|         ||Douglas Miller||         ||Clinical Professor||         ||School of Medicine|| | email@example.com|
|         ||   ||         ||Department of Pathology and Anatomical Sci.||         ||      ||         |
|         ||Kevin F Staveley-O'Carroll||         ||Chair||         ||School of Medicine|| | firstname.lastname@example.org|
|         ||   ||         ||Department of Surgery||         ||      ||         |
|         ||Peter Tonellato||         ||Professor of Bioinformatics||         ||School of Medicine|| | email@example.com|
|         ||   ||         ||Health Management and Informatics||         ||      ||         |
|         ||Nathan Bivens||         ||Director, DNA Core Facility||         ||Office of Research|| | firstname.lastname@example.org|
|         ||   ||         ||Research Core Facilities||         ||      ||         |