The DNA Core Facility, using the ABI 3730xl DNA Analyzer, has the capability to rapidly size DNA fragments, such as, microsatellites (random 2-6 base pair repeats) and AFLPs (amplified fragment length polymorphisms). The ABI 3730xl DNA Analyzer allows for the use of a fluorescence-based detection system that automates the process. Fluorescent dyes are incorporated into the DNA fragment using fluorescence-labeled primers. An internal lane standard that ranges from 20-600 bases (Genescan 600 LIZ) is added to each sample for precise sizing of each fragment adjusting for lane to lane variation. A distinct advantage to the fluorescence-based system is the availability of four dyes allowing the multiplexing of multiple samples in a single lane. Eight samples can reliably be multiplexed in each well of a 96-well plate allowing hundreds of loci to be analyzed in a single day.
Turn around time for single tube submissions is 48-36 hours. A 96-well plate submission turn around time is 24 hours.