Initiating Illumina Sequencing Services


The DNA Core Facility provides high-throughput DNA sequencing services to all four campuses of the University of Missouri system and external researchers. The facility has performed next generation sequencing services since 2008. Core staff are experienced with RNA/DNA QC techniques, library construction methods, and data generation on Illumina instruments. The facility maintains a NextSeq 500 and two MiSeq instruments, as well as, necessary ancillary equipment. The combined data yield of these instruments allows the DNA Core to provide sequencing capacity for both the small and large project.

The DNA Core currently provides library construction services for several methods.

  • Illumina Small RNA Library
  • Illumina mRNA Stranded Library(RNA-Seq)
  • Clonetech Ultra Low Input RNA Library(RNA-Seq)
  • ChIP-Seq Library
  • Illumina DNA PCR-free Library
  • NEB Whole Genome Bisulfite Sequencing (WGBS)
  • NEB Methylated DNA Enrichment Library
  • Amplicon Library
  • 16S Sequencing

The DNA Core offers free consultation for researchers in the grant application process or project initiation phase. Staff can discuss current technology capabilities, prepare cost estimates, and provide letters of support. Additional literature and information on applications, as well as selected publications, can be obtained from the Illumina website.

Contact the DNA Core for additional information on conducting a sequencing project with the facility.





Sample Submission Guidelines
General Comments
  • Advance arrangements should be made before dropping samples off at the lab.
  • Submit samples only after paperwork has been completed.
  • Clearly label tubes.
  • Low binding (siliconized) microcentrifuge tubes (0.5 or 1.5mL) are preferred for storing high quality nucleic acids.
Sample Submission for Library Preparation

total RNA for transciptome profiling (RNA-Seq)
  • Total RNA submitted at a concentration of 100ng/ul in nuclease free water; minimum volume of 15ul (1.5ug total).
  • Indicate specific concentration and method used (OD, Qubit, etc.).
  • Provide sample extraction information as to what methods were used to isolate the sample. This information can help core staff to evaluate the sample quality and trouble shoot any potential problems.
total RNA for small RNA analysis
  • Total RNA submitted at a concentration of 200ng/ul in nuclease free water; minimum volume of 10ul (2.0ug total).
  • Enriched RNA samples for small RNA library preparation may also be submitted. Discuss submission requirements with the DNA Core.
  • Indicate specific concentration and method used (OD, Qubit, etc.).
  • Provide sample extraction information as to what methods were used to isolate the sample. This information can help core staff to evaluate the sample quality and trouble shoot any potential problems.
DNA
  • Genomic DNA submitted at a concentration of 80 ng/ul in 10mM Tris-Cl buffer (pH 8.5, or EB buffer from Qiagen kit ); minimum volume of 40 ul (~3 ug total). Lower concentrations are permissible but must be discussed with core staff before submission.
  • Indicate specific concentration and method used (OD, Qubit, etc.).
  • Provide sample extraction information as to what methods were used to isolate the sample. This information can help core staff to evaluate the sample quality and trouble shoot any potential problems.
Submission of Sequence Ready Libraries
  • 20ul of 10nM stock library
  • Indicate the type of library, fragment size and adapters used.
  • Fragment size profile generated by Agilent Bioanalyzer (or similar instrument).
  • A Qubit quantitation assay will be performed on each library. ($3.50 per sample)