The mission of the University of Missouri DNA Core Facility is to provide genomic services to UM system investigators, external academic researchers and commercial entities in the most cost-effective and timely manner. The facility is a resource to educate and train researchers/students in the use of sequencing technologies with a focus toward implementation of emerging technologies to enhance future research capabilities.
Illumina Sequencing Services
Core staff are experienced with RNA/DNA QC techniques, library construction methods, and data generation on Illumina instruments. The facility maintains a NextSeq 500 and two MiSeq instruments, as well as, necessary ancillary equipment for library preparation. The data yield of instruments allows for a variety of applications and projects.
10x Genomic Services
The 10x Genomics Chromium system provides automated barcoding and library construction for a multitude of transcriptomic and genomic sequencing applications.
Oxford Nanopore Sequencing Services
The Oxford Nanopore Technology (ONT) GridION Mk1 offers the ability to sequence a variety of sample types (i.e., genomic DNA, full-length cDNA, PCR amplicons, direct RNA). Unlike short read technology, the maximum length of a read is solely dependent on the prepared DNA fragment. Average read lengths of >15 kb are routine.
Metagenomic/Targeted Amplicon Services
Several approaches are offered for evaluating and understanding complex microbial communities. Methods include whole genomic sequencing, metatranscriptomics and targeted sequencing of loci (16S, ITS, etc.).
Sanger Sequencing Services
A 3730xl 96-capillary DNA Analyzer using Applied Biosystems Big Dye Terminator cycle sequencing chemistry provides read lengths of 1,100 bases per reaction with high quality template. Turn around time for submissions is < 24 hours.
Fragment Analysis Services
The ABI 3730xl DNA Analyzer has the capability to rapidly size microsatellites (random 2-6 base pair repeats) and AFLPs (amplified fragment length polymorphisms). An internal lane standard that ranges from 20-600 bases (Genescan 600 LIZ) is added to each sample for precise sizing of fragments and adjusting for lane to lane variation. Four dyes allow the multiplexing of multiple samples in a single lane.
Enzyme Freezer Program
The Enzyme Freezer Program stocks an extensive inventory of restriction enzymes, PCR and qPCR reagents, DNA and RNA purification kits, and various chemicals and biochemicals at discount prices and with no shipping charges.