MU DNA Core Facility

The University of Missouri DNA Core Facility is a state of the art research facility providing services to greater than 200 investigators on all four campus of the UM system, outside academic institutions and commercial enterprises. Staff at the DNA Core are eager to provide our services, as well as, our expertise in support of your research.

Illumina Sequencing Services

The MU DNA Core has provided next generation sequencing services since 2008. Core staff are experienced with RNA/DNA QC techniques, library construction methods, and data generation on Illumina instruments.

The facility maintains a NextSeq 500 and two MiSeq instruments, as well as, necessary ancillary equipment. The combined data yield of these instruments allows the DNA Core to provide sequencing capacity for both the small and large project. Contact the DNA Core to discuss a future project.

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Sanger Sequencing Services

The MU DNA Core accommodates both single sample users and high-throughput projects. The facility utilizes a 3730xl 96-capillary DNA Analyzer with Applied Biosystems Big Dye Terminator cycle sequencing chemistry. The 96-capillary instrument with robotic plate handler provides a high throughput capacity at minimal cost to investigators.

Read lengths up to 1,100 bases per reaction are routine, providing the template is of high quality. Turn around time for submissions is 24 hours.

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Stockroom

The DNA Core maintains the campus Enzyme Freezer Program which contains an extensive inventory of restriction enzymes, PCR and qPCR reagents, DNA and RNA purification kits, and various chemicals and biochemicals all at discount prices and with no shipping charges. To learn about stocked items, select "Enzyme Freezer Program" from the menu.

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Fragment Analysis Services

The MU DNA Core, using the ABI 3730xl DNA Analyzer, has the capability to rapidly size DNA fragments, such as, microsatellites (random 2-6 base pair repeats) and AFLPs (amplified fragment length polymorphisms). An internal lane standard that ranges from 20-600 bases (Genescan 600 LIZ) is added to each sample for precise sizing of each fragment adjusting for lane to lane variation. Four dyes allow the multiplexing of multiple samples in a single lane.

Turn around time for single tube submissions is 48-36 hours. A 96-well plate submission turn around time is 24 hours.

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